Product Chambers


Our chambers carry a variety of critical features for tissue growth and 3-D culture.

Our bioreactor chambers are third generation designs, and capture the benefits of customer feedback. Chambers can be tailored to grow/culture different tissues such as: blood vessels, ligaments, cartilage and bone by stimulating cell-seeded scaffolds via perfusion or controlled mechanical deformation. Though each has unique features, all retain common design characteristics required to make your experiments productive:

  • Bioinert materials
  • Compact chamber size
  • Minimized interior volume - small media volumes
  • Autoclavable parts
  • Simple connectors and minimized part: quick assembly
  • Grip fixation for chamber transport
  • Transparent windows for easy construct viewing
  • FDA approved silicone o-ring seals eliminate contamination
  • Supports a variety of scaffold materials and cells

LumeGen V60 Chambers

The LumeGen V60 Series of vascular chambers facilitate pulsatile and shear stress stimulation to a single vascular construct. Standard construct sizes may range from 2 - 6 mm inner diameter and from 10 - 60 mm long. The modular design allows installation of the scaffold and cell seeding independently of the stimulation system. Multiple ports are available for media access, catheter probe insertion, sample collection, or further customization. The chamber has a media volume of 60mL.

Chamber Sample Number Max Sample Size Chamber Volume
V60-1r 1 60mm long x 6mm ID 60ml

 

LigaGen L30 Chambers

The LigaGen L30 Series of Chambers delivers oscillatory compressive/tensile axial stimulation to samples with a large aspect ratio.  The chambers can be used with a variety of construct materials from decellularized tendons and ligaments to polymerized hydrogels. The chamber accommodates samples up to 30 mm long, and 3 mm thick. Multiple grip styles allow for a wide range of construct geometries to be stimulated.  A unique seal proves a mechanical feed-through into the chamber, while facilitating axial motion in a sterile environment with minimal resistance.  Two different chamber designs allow either single sample or multi-sample stimulation.  Both chambers can be used with a perfusion system to provide convective media transport around the sample.


Chamber Sample Number Max Sample Size Chamber Volume
L30-1X 1 30mm long x 3mm thick 23 ml
L30-4C 2 or 4 30mm long x 3mm thick 80 ml
L150-1 1 150mm long x 5mm thick ~75ml

 

Grip Chamber Availability Features
40N Spring Assisted Wedge Action Grip
L30-1X, L30-4C 4.8mm wide wedge shaped grip faces with gentle convolutions designed to secure slippery tissue. Accommodates samples 3mm thick and 4mm wide.
40N Hydrogel Grips
L30-1X Zero-clamp design includes an integrated construct mold  to enable mechanical stimulation of hydrogel scaffolds
40N Screw Action Grips
L30-1X, L150-1 Flat faces with serrated surfaces, provide a simple and efficient method to secure biological tissue. Available in 6mm wide or 10mm wide configurations.
Custom Grips L30-1X, L30-4C Custom designed for specific scaffold textures or geometries

 

CartiGen C10 Chambers

The CartiGen C10 Series of Chambers delivers oscilatory compressive stimulation4 to disc shaped samples. The chambers can be used with a variety of construct geometries by choosing one of our many sample platen options, maximum sample size per individual well is 10 mm diameter by 5 mm thick.  The maximum sample size for single sample chamber is 30 mm diameter by 5 mm thick. A unique seal provides a mechanical feed-through into the chamber while facilitating axial motion in a sterile environment while allowing axial motion with minimal resistance. Perfusion through a single construct is available with porous platens.  The C10-12c chamber has optical grade windows on the bottom, allowing researchers to visualize their samples in real time, via confocal microscopy, without interrupting the culture.

Chamber Sample Number Max Sample Size Chamber Volume
C10-1 1 30mm diameter x 5mm thick 10 ml
C10-12c max 12 10mm diameter x 5mm thick 2ml/well

 

Platen Chamber Availability Features
Smooth C10-1, C10-12c Autoclavable polypropylene, smooth flat face construction.
Porous C10-1, C10-12c Sintered stainless steel.  Enables perfusion through the platen and into construct bottom face.
Hydrogel C10-1, C10-12c Porous polymer design allows the sample to be cast into platens, preventing the gel from moving.
Custom C10-1, C10-12c A modified version of BISS's standard platen, built to your specification.

 

OsteoGen O10 Chambers

The OsteoGen O10 Series of Chambers deliver perfusion5 and hydrostatic pressures to a cell seeded cylindrical scaffold. The chamber accommodates samples up to 10 mm diameter and 10 mm long. The translucent polymer housing allows visualization of the construct and media during culture.

Chamber Sample Number Max Sample Size Chamber Volume
O10-1 1 10mm diameter x 10mm long 2 ml

 

DermiGen D70 Chambers

The DermiGen D70-1 Series of chambers delivers oscillatory tensile axial stimulation to samples with a large aspect ratio. The chambers can be used with a variety of construct materials. The chamber accommodates samples up to 70 mm long, 20mm wide, and 2 mm thick.  The chamber body's unique design provides an air/media interface. The chamber assists media perfusion/flow along the underside of the construct while deterring media from spilling onto the topside. Custom mechanical grips with a soft lining provide secure positioning of the sample. A unique seal provides a mechanical feed-through into the chamber, while facilitating axial motion in a sterile environment with minimal resistance.


Chamber Sample Number Max Sample Size Chamber Volume*
D70-1 1 70mm long x 20mm wide x 2mm thick 15 ml

 

*Chamber volume reported assumes chamber is being used with air/media interface

 


References:
1. Niklason, L., Science, 1999, Nov 19; 286(5444):1493-4.
2. Vunjak-Novakovic, G., et al., Annu Rev Biomed Eng. 2004; 6:131-56.
3. Mauck, R. et al., J Biomech Eng, 2000 Jun; 122(3):252-60.
4. Glowacki, J., et al., Cell Transplant., 1998,  May-Jun;7(3):319-26.

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